Guinea Pig Serum l-Asparaginase
نویسندگان
چکیده
منابع مشابه
Guinea pig serum L-asparaginase. Properties, purification, and application to determination of asparagine in biological samples.
After the first isolation of asparagine in 1806 by Vauquelin and Robiquet (l), there were numerous studies of its properties and physiological significance (2-4). Piria (5) in 1848 was probably the first to adduce evidence for a physiological role of asparagine during the life cycle of plants, and also to observe its transformation or metabolism by yeast. However, it was not until approximately...
متن کاملEvidence That the L-asparaginase of Guinea Pig Serum Is Responsible for Its Antilymphoma Effects
A number of the properties of the L-asparaginase present in guinea pig serum have been examined and shown to be indistinguishable from those of the agent responsible for inhibiting cells of lymphoma 6C3HED in vivo. The patterns of instability of the enzyme to changes in temperature and pH were found to parallel closely those of the antilymphoma agent. L-Asparaginase activity was essentially abs...
متن کاملIn Vivo and in Vitro Effects of the L-Asparaginase Fraction of Guinea Pig Serum on the 6C3HED Ascites Lymphosarcoma1
The antitumor activity of guinea 1'ig serum fractions was studied in vivo and in vitro by bright field, fluorescence, and electron microscopy observations and by dye permeability and cytidine-3H incorporation in 6C3HED lymphosarcoma cells. In C3H mice bearing the ascites tumor treated with the Lasparaginase fraction, there was a decrease in the total num ber and mitosis of tumor cells, a reduct...
متن کاملIn vivo and in vitro effects of the L-asparaginase fraction of guinea pig serum on the 6C3HED ascites lymphosarcoma.
The antitumor activity of guinea 1'ig serum fractions was studied in vivo and in vitro by bright field, fluorescence, and electron microscopy observations and by dye permeability and cytidine-3H incorporation in 6C3HED lymphosarcoma cells. In C3H mice bearing the ascites tumor treated with the Lasparaginase fraction, there was a decrease in the total num ber and mitosis of tumor cells, a reduct...
متن کاملStructural and Kinetic Characterization of Guinea Pig l-Asparaginase Type III
We investigated whether an uncharacterized protein from guinea pig could be the enzyme behind Kidd's serendipitous discovery, made over 60 years ago, that guinea pig serum has cell killing ability. It has been long known that an enzyme with l-asparaginase activity is responsible for cell killing, although astonishingly, its identity remains unclear. Bacterial asparaginases with similar cell kil...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1963
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)81247-9